Alcohol produces changes in behavior through molecular effects on ion channels, enzymes and transporters.
Many proteins have been elucidated that at least in part mediate behavioral changes induced by alcohol.
Among them, 53 enzymes were determined to be likely involved in fluoranthene degradation.
We integrated the metabolic information with the genomic and proteomic results and proposed pathways for the degradation of fluoranthene.
They are also linked to diseases including neurodegenerative diseases (Parkinson’s and Alzheimer’s), cancer and diabetes.
The importance of these proteins has resulted in substantial interest from the biophysics and biology communities.
In both reactions, loose association of the NADP(H) was followed by two conformational changes, which increased cofactor affinity by (ratio of the maximum rates of single turnover) were 1.06 and 2.06, respectively.
According to our hypothesis, the oxidation of fluoranthene is initiated by dioxygenation at the C-1,2, C-2,3, and C-7,8 positions.
The C-1,2 and C-2,3 dioxygenation routes degrade fluoranthene via fluorene-type metabolites, whereas the C-7,8 routes oxidize fluoranthene via acenaphthylene-type metabolites.
Since the flexibility of these proteins also makes them hard to study using traditional structural biology methods, development and application of new methods or analyses (such as single-molecule experiments in our lab) is also an important line of work in the field.
Alpha-synuclein is an important example of an IDP that has been studied quite extensively for several years.